Tryptophan Synthase: Biocatalyst Extraordinaire
نویسندگان
چکیده
منابع مشابه
A centenarian extraordinaire.
Henry Rollin, a distinguished Member, Fellow and now Honorary Fellow of the Royal College of Psychiatrists, has attained his 101st year. The achievements of this remarkable man are described and speculations made about the reasons for his longevity and his plans for the future.
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The tryptophan synthase genes, trpA and trpB, of Bacillus stearothermophilus IFO13737 were cloned by transformation of tryptophan auxotrophic mutations of the trp genes into Escherichia coli. The genes are located in the order of trpB and tipA, according to their coding orientation, in a 2.5 kb EcoKV-Hindlll DNAfragment. The complete nucleotide sequence of this DNAwas determined. The tipA and t...
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To probe the structural and functional roles of activesite residues in the tryptophan synthase a2b2 complex from Salmonella typhimurium, we have determined the effects of mutation of His in the b subunit. His is located adjacent to b subunit Lys, which forms an internal aldimine with the pyridoxal phosphate and catalyzes the abstraction of the a-proton of L-serine. The replacement of His by leu...
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ژورنال
عنوان ژورنال: ChemBioChem
سال: 2020
ISSN: 1439-4227,1439-7633
DOI: 10.1002/cbic.202000379